Fungi can grow anywhere over a wide-ranging temperature with sufficient nutrients and moisture . Therefore, indoor mold problem has long existed in yearlong warm and humid countries like Malaysia and other Southeast Asian countries. In previous research, airborne fungi growth was found in a high humid room (relative humidity ~87%) in a higher educational building of computer studies after conventional remediation such as detergent-cleaning and changing of the affected ceiling boards . It is dangerous since researchers suggested that the chances of transmitting the contaminating microbes through using of computers in a university setting is potentially great due to the enormous usage of computer keyboards that are not routinely sanitized by numerous users daily . The study also shows that the conventional remediation is not a long term solution to circumvent the indoor airborne fungal contamination. However, green solutions are needed to reduce the viability of the airborne fungi and thereby the existence of secondary metabolites of fungi in indoor setting in order to secure the quality of teaching and learning among educators and students in a university building.Lately, a bioactive compound from food industry, potassium sorbate had been shown to be able to control the growth of two fungus species (Chaetomium globosum and Alternaria alternate) isolated from an indoor waterborne coating . It is suggested to be effective against airborne fungi too. Therefore, this study aimed to evaluate the ability of potassium sorbate as a biocide to render the viability of indoor airborne fungi in a higher educational building of computer studies of a university located at Southern Peninsular Malaysia.